Single event visualization of unconventional secretion of FGF2

Dimou E, Cosentino K, Platonova E, Ros U, Sadeghi M, Kashyap P, Katsinelos T, Wegehingel S, Noé F, García-Sáez AJ, Ewers H,and Nickel W (2018)

J Cell Biol ( PMID:  30470711)

Quantitative analysis of circadian single cell oscillations in response to temperature

Abraham U, Schlichting JK, Kramer A, Herzel H.

PLoS One. 2018 Jan 2;13(1):e0190004. doi: 10.1371/journal.pone.0190004.

Ultradian Rhythms in the Transcriptome of Neurospora crassa

Ananthasubramaniam B, Diernfellner A, Brunner M, Herzel H.

iScience. 2018 Nov 30;9:475-486. doi: 10.1016/j.isci.2018.11.012. Epub 2018 Nov 10.

Regulation of synaptic release-site-Ca2+-channel coupling as a mechanism to control release probability and short-term plasticity

Bohme, M. A., Grasskamp, A. T., and Walter, A. M.

FEBS Lett doi:10.1002/1873-3468.13188

Synaptotagmin-1 drives synchronous Ca2+-triggered fusion by C2B-domain-mediated synaptic-vesicle-membrane attachment.

Chang S, Trimbuch T, Rosenmund C.

Nat Neurosci. 2018 Jan;21(1):33-40. doi: 10.1038/s41593-017-0037-5. Epub 2017 Dec 11.

Unconventional mechanisms of eukaryotic protein secretion

Dimou E and Nickel W.

Curr Biol 28:R406-R410

Open-source recombinant monoclonal secondary

Ewers H

Nanobodies J Cell Biol, 217 (3) 809-11

Optical tools for understanding the complexity of beta cell signaling and insulin release

Frank, J., Broichhagen, J., Yushchenko, D., Trauner, D., Schultz, C., Hodson, D.

Nat Rev Endocrinol. 2018 Dec;14(12):721-737. doi: 10.1038/s41574-018-0105-2.

Surface Immobilization of Virus and Nanoparticles Elucidates Early Events in Clathrin-mediated Endocytosis

Fratini, Marta, Tina Wiegand, Charlotta Funaya, Joachim P. Spatz, Elisabetta Ada Cavalcanti-Adam, Steeve Boulant.

ACS Infect Dis. 2018 Nov 9;4(11):1585-1600. doi: 10.1021/acsinfecdis.8b00134. Epub 2018 Sep 26.

Expansion Stimulated Emission Depletion Microscopy (ExSTED)

Gao M, Maraspini R, Beutel O, Zehtabian A, Eickholt B, Honigmann A, and Ewers H.

ACS Nano. 2018 May 22;12(5):4178-4185. doi: 10.1021/acsnano.8b00776. Epub 2018 Apr 19.

Endogenous Fatty Acids are Essential Autocrine Signaling Factors of Pancreatic β-Cells and Insulin Secretion

Hauke, S., Keutler, K., Phapale, P., Yushchenko, D., Schultz, C.

Diabetes 67.

The Evolution of Variability and Robustness in Neural Development

Hiesinger, P.R. and Hassan, B.A.

Trends in Neuroscience 41(9):577-586

The where, what, and when of membrane protein degradation in neurons

Jin EJ, Kiral FR, Hiesinger PR.

Dev Neurobiol. 2018 Mar;78(3):283-297. doi: 10.1002/dneu.22534. Epub 2017 Sep 19. Review.

Live observation of two parallel membrane degradation pathways at axon terminals

Jin, E.J., Kiral, F.R., Özel, M.N., Burchardt, L.S., Osterland, M., Epstein, D., Wolfenberg, H., Prohaska, S., and Hiesinger, P.R.

Curr. Biol., 28(7):1027-1038.e4

PTEN suppresses axon outgrowth by down-regulating the level of detyrosinated microtubules

Kath, C., Mueller, R., Schultz, C., Haucke, V., Eickholt, B., Schmoranzer, J.

PLoS One 13, e0193257 (2018). doi: 10.1371/journal.pone.0193257.

Unconventional secretion mediates the trans-cellular spreading of tau

Katsilenos, T, Zeitler M, Dimou E, Karakatsani A, Müller HM, Nachman E, Steringer JP, Ruiz de Almodovar C, Nickel W, Jahn TR.

Cell Rep 23:2039-2055.

Rab GTPases and Membrane Trafficking in Neurodegeneration

Kiral, F.K., Kohrs, F., Jin, E.J. and Hiesinger, P.R.

Curr Biol. Apr 23;28(8):R471-R486 (

Rab GTPases and Membrane Trafficking in Neurodegeneration

Kiral, F.K., Kohrs, F., Jin, E.J. and Hiesinger, P.R.

Curr Biol. Apr 23;28(8):R471-R486 (

A potent and selective PARP11 inhibitor suggests coupling between cellular localization and catalytic activity

Kirby, I. T., Kojic, A., Arnold, M. R., Thorsell, A.-G., Karlberg, T., Vermehren-Schmaedick, A., Sreenivasan, R., Schultz, C., Schüler, H., Cohen M. S.

Cell Chem Biol. 2018 Dec 20;25(12):1547-1553.e12. doi: 10.1016/j.chembiol.2018.09.011. Epub 2018 Oct 18.

Novel lipid tools and probes for biological investigations

Laguerre, A., Schultz, C.

Curr Opin Cell Biol. 2018 Aug;53:97-104. doi: 10.1016/ Epub 2018 Jul 14. Review.

VAMPnets for deep learning of molecular kinetics

Mardt, Andreas, Luca Pasquali, Hao Wu & Frank Noé.

Nature Communicationsvolume 9, Article number: 5 (2018) (

Inhibition of calpain-1 stabilizes TCF11/Nrf1 but does not affect its activation in response to proteasomal inhibition

Nowak K, Taubert RM, Haberecht S, Venz S, Krüger E.

Biosci Rep. 2018 Sep 3. pii: BSR20180393. doi: 10.1042/BSR20180393. [Epub ahead of print]

Control of SUMO and Ubiquitin by ROS: Signaling and disease implications.

Stankovic-Valentin N, Melchior F.

Mol Aspects Med. 63, 3-17. (Review)

Autoregulation of class II alpha PI3K activity by its lipid binding PX-C2 domain module

Wang, H., Lo, W.T., Vujičić Žagar, A., Gulluni, F., Scapozza, L., Haucke, V.*, Vadas, O.*

Mol. Cell, 71, 343-351(*co-corresponding authors)

Phosphoinositide conversion in endocytosis and in the endolysosomal system

Wallroth, A., Haucke, V.

J Biol. Chem. 293, 1526-1535 [doi: 10.1074]

Deep Generative Markov State Models

Wu, Hao, Andreas Mardt, Luca Pasquali and Frank Noe.

32nd Conference on Neural Information Processing Systems (NeurIPS 2018), Montréal, Canada

An emerging case for membrane pore formation as a common mechanism for the unconventional secretion of FGF2 and IL-1beta

Brough D, Pellegrin P, and Nickel W.

J Cell Sci 130:3197-3202.

The coding and noncoding transcriptome of Neurospora crassa

Cemel IA, Ha N, Schermann G, Yonekawa S, Brunner M.

BMC Genomics, 18(1), 978, doi: 10.1186/s12864-017-4360-8.

Optical control of GPR40 signalling in pancreatic ß-cells

Frank, J. A., Yushchenko, D. A., Fine, N. H. F., Duca, M., Citir, M., Broichhagen, J., Hodson, D. J., Schultz, C., Trauner, D.

Chem. Sci. 8, 7604-7610 (2017). doi: 10.1039/c7sc01475a.

Trifunctional lipid probes for comprehensive studies of single lipid species in living cells

Höglinger, D., Nadler, A., Haberkant, P., Kirkpatrick, J., Schifferer, M., Stein, F., Hauke, S., Porter, F. D., Schultz, C.

Proc. Natl. Acad. Sci. USA 114, 1566-1571.

Reciprocal regulation of carbon monoxide metabolism and the circadian clock.

Klemz R, Reischl S, Wallach T, Witte N, Jürchott K, Klemz S, Lang V, Lorenzen S, Knauer M, Heidenreich S, Xu M, Ripperger JA, Schupp M, Stanewsky R, Kramer A.

Nat Struct Mol Biol. 2017 Jan;24(1):15-22

Thiolutin is a zinc chelator that inhibits the Rpn11 and other JAMM metalloproteases.

Lauinger L, Li J, Shostak A, Cemel IA, Ha N, Zhang Y, Merkl PE, Obermeyer S, Stankovic-Valentin N, Schafmeier T, Wever WJ, Bowers AA, Carter KP, Palmer AE, Tschochner H, Melchior F, Deshaies RJ, Brunner M, Diernfellner A.

Nat Chem Biol. 2017 Jul;13(7):709-714. doi: 10.1038/nchembio.2370. Epub 2017 May 1.

mTORC1 activity repression by late endosomal phosphatidylinositol 3,4-bisphosphate.

Marat AL, Wallroth A, Lo WT, Müller R, Norata GD, Falasca M, Schultz C, Haucke V.

Science. 2017 Jun 2;356(6341):968-972. doi: 10.1126/science.aaf8310.


Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function.

Piwecka M, Glažar P, Hernandez-Miranda LR, Memczak S, Wolf SA, Rybak-Wolf A, Filipchyk A, Klironomos F, Cerda Jara CA, Fenske P, Trimbuch T, Zywitza V, Plass M, Schreyer L, Ayoub S, Kocks C, Kühn R, Rosenmund C, Birchmeier C, Rajewsky N.

Science. 2017 Sep 22;357(6357). pii: eaam8526. doi: 10.1126/science.aam8526. Epub 2017 Aug 10.

Stable Positioning of Unc13 Restricts Synaptic Vesicle Fusion to Defined Release Sites to Promote Synchronous Neurotransmission.

Reddy-Alla S, Böhme MA, Reynolds E, Beis C, Grasskamp AT, Mampell MM, Maglione M, Jusyte M, Rey U, Babikir H, McCarthy AW, Quentin C, Matkovic T, Bergeron DD, Mushtaq Z, Göttfert F, Owald D, Mielke T, Hell SW, Sigrist SJWalter AM.

Neuron. 2017 Sep 13;95(6):1350-1364.e12. doi: 10.1016/j.neuron.2017.08.016. Epub 2017 Aug 31.

Flavivirus infection uncouples translation suppression from cellular stress responses

Roth H, Magg V, Uch F, Mutz P, Klein P, Haneke K, Lohmann V, Bartenschlager R, Fackler OT, Locker N, Stoecklin G, Ruggieri A.

mBio 8(1):e02150-­‐16.

A Ratiometric Sensor for Imaging Insulin Secretion in Single β Cells

Schifferer, M., Yushchenko, D. A., Hodson, D. J., Bolbat, A., Stein, F., Schultz, C.

Cell Chem. Biol. 24, 525-531 (2017).

Key steps in unconventional secretion of fibroblast growth factor 2 reconstituted with purified components.

Steringer JP, Lange S, Čujová S, Šachl R, Poojari C, Lolicato F, Beutel O, Müller HM, Unger S, Coskun Ü, Honigmann A, Vattulainen I, Hof M, Freund C, Nickel W.

Elife. 2017 Jul 19;6. pii: e28985. doi: 10.7554/eLife.28985

Principles for circadian orchestration of metabolic pathways.

Thurley K, Herbst C, Wesener F, Koller B, Wallach T, Maier B, Kramer A, Westermark PO.

Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1572-1577

A lipid off-switch for mTORC1

Wallroth, A., Haucke, V.

Mol. Cell. Oncol. 4:e1356899

Phosphatidylinositol 4,5-bisphosphate optical uncaging potentiates exocytosis.

Walter AM, Müller R, Tawfik B, Wierda KD, Pinheiro PS, Nadler A, McCarthy AW, Ziomkiewicz I, Kruse M, Reither G, Rettig J, Lehmann M, Haucke V, Hille B, Schultz C, Sorensen JB.

Elife. 2017 Oct 25;6. pii: e30203. doi: 10.7554/eLife.30203.

Optical control of protein translocation with a photoswitchable diacylglycerol

Frank, J. A., Yushchenko, D. A., Hodson, D. J., Lipstein, N., Nagpal, J., Rutter, G. A., Rhee, J.-S., Gottschalk, A., Brose, N., Schultz*, C., Trauner*, D.

Nat. Chem. Biol. 12, 755-762 (2016), doi:10.1038/nchembio.2141

Small Molecule Inhibitors Targeting Tec Kinase Block Unconventional Secretion of Fibroblast Growth Factor 2.

La Venuta G, Wegehingel S, Sehr P, Müller HM, Dimou E, Steringer JP, Grotwinkel M, Hentze N, Mayer MP, Will DW, Uhrig U, Lewis JD, Nickel W.

J Biol Chem. 2016 Aug 19;291(34):17787-803. doi: 10.1074/jbc.M116.729384. Epub 2016 Jul 5.

Feedback Loops of the Mammalian Circadian Clock Constitute Repressilator.

Pett JP, Korenčič A, Wesener F, Kramer A, Herzel H.

PLoS Comput Biol. 2016 Dec 12;12(12):e1005266.

MHC class II complexes sample intermediate states along the peptide exchange pathway

Wieczorek, M., J. Sticht, S. Stolzenberg, S. Günther, C. Wehmeyer, Z. El Habre, M. Alvaro-Benito, F. Noé*, C. Freund*.

Nature Communications 7, 13224 (2016)