Prof. Dr. Francesca Bottanelli

Francesca Bottanelli

Freie Universität Berlin
Institut für Chemie und Biochemie
Thielallee 63
14195 Berlin, Germany
Phone: +49 (30) 838 65860

Research Area

Cell Biology, super-resolution imaging

Position and Status

Junior Professor of Biochemistry (W1)

Scholarships and awards

2012-2014  American-Italian Cancer Foundation post-doctoral fellowship  
2006-2010  Leeds university international research PhD scholarship  

University training and degree

2006 - MSc in Plant, Food and Environmental Biotechnology, University of Milan (IT)
10/2004 - 09/2005 - MSc thesis work at the University of Leeds (UK). Supervisor: Dr. J. Denecke.
01/2004 – 04/2004 - BSc thesis work at the University of Milan (IT). Supervisor: Dr. Gabriella Consonni.


Advanced academic qualifications

2011 - PhD in Biology, Faculty of Biological Sciences, The University of Leeds (UK). Supervisor: Dr. J. Denecke.


Postgraduate professional career

09/2018 - present - Junior professor, Freie Universitä Berlin, Institut für Chemie und Biochemie, Biochemie.  
04/2011 - 06/2018 - Post-doctoral fellow, Yale University School of Medicine. Cell Biology department (USA). Advisor: Dr. J. Rothman.

Coordinating functions and editorial work

2019 - Present - Member of the executive committee for SFB958 “Scaffolding of Membranes - Molecular Mechanisms and Cellular Functions“.

Most important publications 

  1. Ernst, A. M., Syed, S. A., Zaki, O., Bottanelli, F., Zheng, H., Hacke, M., Xi, Z., Rivera-Molina, F., Graham, M., Rebane, A. A., Björkholm, P., Toomre, D., Baddeley, D., Pincet, F. and Rothman J.E. (2018) S- Palmitoylation Sorts Membrane Cargo for Anterograde Transport in the cis-Golgi. Dev. Cell. 19;47(4):479-493.e7

  2. Kory, N., Wyant, G.A., Prakash, G., uit de Bos, J., Bottanelli, F., Pacold, M.E., Chan, S.H., Lewis, C.A., Wang, T., Keys, H.R., Guo, Y.E., Sabatini D.M. (2018) SFXN1 is a mitochondrial serine transporter required for one-carbon metabolism. Science. 362(6416), p.eaat9528

  3. Bottanelli, F., and Schroeder, L.K. (2018) Stimulated Emission Depletion (STED) imaging of clathrin- mediated endocytosis in living cells. Methods Mol Biol. 2018;1847:189-195. doi: 10.1007/978-1-4939- 8719-1_14

  4. Bottanelli, F., Kilian N., Ernst, A.M., Rivera-Molina, F., Schroeder, L.K., Kromann, E.B., Lessard, M.D., Erdmann, R.S., Schepartz, A., Baddeley, D., Bewersdorf, J. , Toomre, D. and Rothman J.E. (2017) A novel physiological role for ARF1 in the formation of bi-directional tubules from the Golgi. Molecular biology of the cell. doi:10.1091/mbc.E16-12-0863

  5. Dancourt, J., Zheng, H., Bottanelli, F., Allgeyer E., Bewesdorf, J., Graham, M., Liu, X., Rothman, J.E., and Lavieu G. (2016) Small Cargoes Pass Through Synthetically Glued Golgi Stacks. FEBS Lett. doi: 10.1002/1873-3468.12210

  6. Bottanelli, F.#, Kromann., E.B.#, Allgeyer E.S., Erdmann, R.S., Baguley, S.W., Sirinakis, G., Schepartz, A., Baddeley, D., Toomre, D.K., Rothman J.E., and Bewersdorf, J. (2016). Two-color live-cell nanoscale imaging of intracellular targets. Nature Communications. 7, 10778. doi:10.1038/ncomms10778

  7. Lavieu, G., Dunlop, M.H., Lerich, A., Zheng, H., Bottanelli, F., and Rothman, J.E. (2014). The Golgi ribbon structure facilitates anterograde transport of large cargoes. Molecular biology of the cell 25, 3028-3036

  8. Schill, H., Nizamov, S., Bottanelli, F., Bierwagen, J., Belov, V.N., and Hell, S.W. (2013). 4- Trifluoromethyl-substituted coumarins with large Stokes shifts: synthesis, bioconjugates, and their use in super-resolution fluorescence microscopy. Chemistry 19, 16556-16565

  9. Bottanelli, F.,Gershlick, D.C., and Denecke, J. (2012). Evidence for sequential action of Rab5 and Rab7 GTPases in prevacuolar organelle partitioning. Traffic 13, 338-354

  10. Bottanelli, F., Foresti, O., Hanton, S., and Denecke, J. (2011). Vacuolar transport in tobacco leaf epidermis cells involves a single route for soluble cargo and multiple routes for membrane cargo. The Plant cell 23, 3007-3025

For a full list of publications: