Quantitative image processing. Fluorescence microscopy is a ubiquitous tool in modern molecular cell biology since the GFP-revolution allowed the observation of the localization and dynamics of proteins in living cells. Consequently, most projects in this consortium employ assays based on fluorescence microscopy and aim to extract quantitative information from these for analysis. We propose here the implementation of a common resource for the development of quantitative image processing routines and for training towards the application of such routines in the participating laboratories. The central project will provide advice on the selection and implementation of existing image-processing resources and develop specific novel image-processing and data analysis routines. Dedicated IT-resources will be generated for the swift exchange of data and remote-guided image analysis coaching for all participating laboratories.